【報告題目】Biocompatible and Photostable AIE Nanoprobes for Multiphoton Functional Bioimaging

【報告人】錢駿博士

【邀請人】賀號博士

【時間】1月16日（周一）10:00-11:00

【地點】Med-X 108

【摘要】Relying on simultaneous absorption of two or more near-infrared photons by a fluorophore, multiphoton microscopy can achieve deep tissue penetration and efficient light detection noninvasively, which makes it very suitable for thick-tissue and in vivo bioimaging. However, most conventional organic fluorophores suffer from severe aggregation-caused quench (ACQ) effect, which means their fluorescence is easily quenched at aggregate or solid state. In 2001, Tang and coworkers observed a diametrically opposite phenomenon called “aggregation-induced emission” (AIE), which provides an ideal solution to the notorious ACQ problem. Based on this mechanism, hydrophobic AIE molecules can be encapsulated with various types of nanoparticles, or form nanoaggregates in an aqueous solution spontaneously. AIE nanoparticles have bright fluorescence, large multiphoton absorption cross-section, and are very resistant to photobleaching even under high-power laser irradiation, which facilitates their applications in long-term multiphoton microscopy as nanoprobes. In this talk, I will introduce some exemplary applications of AIE nanoparticles in two- and three-photon in vivo functional brain imaging of mice, such as brain-microglia imaging, 3D architecture reconstruction of brain blood vessel, and blood velocity measurement. We also applied AIE nanoparticles in three-photon imaging of zebrafish at different growth stages.

【報告人簡介】錢駿，博士，男，浙江大學光電科學與工程意昂4教授，博士生導師🚢。2004年6月在浙江大學光電系獲學士學位🧜🏽，2009年6月在浙江大學光電系獲博士學位，2009年7月至2011年6月在浙江大學光及電磁波研究中心從事博士後研究，2011年6月至2011年12月在浙江大學光電系擔任講師，2012年1月至2015年12月在光電系擔任副教授🍮，擔任博士生導師，2016年1月至今在光電意昂4擔任教授🕊。2006年至2007年曾在美國紐約州立大學布法羅分校“激光、光子學、生物光子學研究中心”進行學術訪問，師從納米生物光子學的國際領軍人物Paras N. Prasad教授。主要從事納米生物光子學的研究。已發表SCI 論文50多篇,其中🙅🏽‍♀️，以第一/通訊作者身份在Adv. Mater., Angew Chem Int Edit, ACS Nano, Biomaterials, Nano Research, Theranostics, Optics. Lett.,Biomed. Opt. Express., J. Biomed. Opt.等高水平期刊上發表論文30多篇🌦。著有英文學術專著一章節。承擔了國家自然科學基金（面上和青年）🪂，973、863子課題👮🏽，浙江省傑出青年科學基金等項目。